Determination of Total Phenolic and Flavonoid Content, Antidiabetic, and Antioxidant Activities of Leaves and Seeds Extracts of Eucalyptus robusta Sm. and Ageratina adenophora Spreng

The present study aimed at the determination of total phenolic and flavonoid content biological activities Eucalyptus robusta (Sm.) Ageratina adenophora (Spreng.) growing in Kathmandu Nepal. People have been using these medicinal plants for many years against infectious diseases to control diabetes. extraction plant secondary metabolites was done by cold percolation method. (TPC) (TFC) were quantified Folin-Ciocalteu phenol reagent aluminium chloride colorimetric α-amylase enzyme inhibition activity performed evaluate antidiabetic property extracts. antioxidant potential extracts evaluated DPPH (2,2-diphenyl- 1-picrylhydrazyl) radical scavenging assay. methanol extract Spreng leaves is found rich a yield percentage 36.83% whereas Sm. seeds 30.75% 27.12% respectively. E. showed highest (200.89±6.67 mg gallic acid equivalent/g dry extract) A. Spreng. (0.49±0.02 quercetin among three samples studied, strongest with half-maximal inhibitory concentration IC50 110.8±1.73 μg/mL. potent towards 16.05±0.24 μg/mL 21.93±1.24 mg/mL 21.82±0.09 This two are compounds. These could be used isolate natural compounds that may drug candidates future discovery process. supports providing scientific validation